- 商品介绍
- 规格参数
- 包装参数
规格 |
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Detection Method: | User defined detection system |
Gel Compatibility: | Bolt™ Bis-Tris Plus Gels, Novex® Tricine Gels, Novex® Tris-Glycine Gels, NuPAGE® Bis-Tris Gels, NuPAGE® Tris-Acetate Gels, SDS-PAGE Gels |
Number of Markers: | 9 |
Product Line: | MagicMark™ |
Shipping Condition: | Dry Ice |
Size Range: | 20 to 220 kDa |
System Type: | Western Blotting, SDS-PAGE |
Kit Contents: | 250μL of ready-to-use standardmixture is provided in a plastic vial; Loading buffer consists of 125mM Tris-HCl (pH 6.8), 10mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue |
Molecular Weight: | 220, 120, 100, 80, 60, 50, 40, 30, 20 kDa |
Stain Type: | Unstained |
Quantity: | 250 μL |
储存 |
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250 µl of ready-to-use standard mixture is provided in a plastic vial. Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue. Store at -20°C. |
描述 |
MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot. The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Compare and view all other protein standards and ladders ›
Applications
• Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using western blot detection method
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