规格
|
Sample Type:
|
Synthetic siRNA |
Cell Type:
|
Established Cell Lines, Primary Cells, Hard-to-Transfect Cells |
High-throughput Compatibility:
|
High-throughput Compatible |
Serum Compatible:
|
No |
Shipping Condition:
|
Wet Ice |
Transfection Technique:
|
Lipid-Based Transfection |
Product Line:
|
Oligofectamine™ |
储存
|
Contains one vial (1 ml) Oligofectamine™ Reagent. Store at 4°C. Do not freeze. |
Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells. Oligofectamine™ Transfection Reagent forms stable complexes with oligos, permitting efficient transfection into eukaryotic cells in a highly specific, yet nontoxic fashion. Oligofectamine™ Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO, HEK-293, NIH 3T3, and HeLa.
Using Oligofectamine™ Transfection Reagent
Oligofectamine™ Reagent is easy to use because it provides a simple and fast protocol. Just dilute Oligofectamine™ Reagent, mix with oligonucleotide, and add to your cells. Oligofectamine™ Reagent requires nanomolar quantities of antisense oligonucleotide, reducing the amount of valuable oligonucleotide needed by up to a thousand-fold. This makes it ideal for high-throughput applications. In addition, Oligofectamine™ Reagent has also been shown to work for siRNA transfections. We recommend this reagent when performing RNAi knockdown experiments in HeLa cells. Please visit RNAi Central for more information.