规格
|
Detection Method:
|
Fluorescent |
Excitation/Emission:
|
482⁄497 |
Flow Cytometer Laser Lines:
|
488 |
Indicator:
|
Membrane Potential Probes |
Indicator Properties:
|
Fluorescent Dye-Based |
Label or Dye:
|
DiOC2(3) |
No. of Reactions:
|
100 |
Product Line:
|
BacLight™ |
Shipping Condition:
|
Wet Ice |
For Use With (Equipment):
|
Flow Cytometer |
Format:
|
Tube(s) |
储存
|
Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 µL of 500 µM solution in DMSO), and PBS (200 mL).
Store in refrigerator (2–8°C) and protect from light. |
The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.
View additional information about all microbiology assays for flow cytometry.