QuantiNova Probe RT-PCR Kit (100)，208352，Qiagen，凯杰

QuantiNova RT-PCR Kits (real-time RT-PCR kits) enable sensitive quantification of RNA targets by real-time one-step PCR using SYBR Green I or sequence-specific probes. A combination of various integrated safety features removes variables and prevents artifacts, ensuring reliable gene expression profiling. The combination of a unique two-phase hot-start and PCR buffer system in the ready-to-use master mix allows room-temperature setup and ensures highly sensitive qRT-PCR on any real-time cycler. The optional QuantiNova Internal Control RNA can be used to test successful reverse transcription and amplification. It is intended to report instrument or chemistry failures, errors in assay setup and the presence of inhibitors. Furthermore, the visual pipetting control can be used to monitor correct pipetting. The integrated gDNA reduction step in the QuantiNova Probe RT-PCR procedure prevents overquantification of transcripts caused by genomic DNA carryover. The QuantiNova Multiplex RT-PCR Kit enables fast and reliable quantification of up to 5 RNA targets in a single tube by multiplex real-time RT-PCR. Our Q-Bond technology and optimized master mix promote ultrafast multiplex real-time RT-PCR within 1 hour. The combination of a unique two-phase hot-start procedure with our multiplex PCR buffer system ensures highly sensitive qRT-PCR on any real-time cycler without the need for optimization, and enables automated reaction setup at room temperature. The kit also provides protocols for extracted RNA and direct amplification from cultured cells, even down to a single cell. The optional QuantiNova Internal Control RNA can be used to monitor successful reverse transcription and amplification, and the visual pipetting control can be used to monitor correct pipetting.

• Unique two-phase hot-start procedure for room-temperature setup
• Visual pipetting control, resulting in fewer pipetting errors
• Internal control for positive in-process verification of successful RT-PCR
• Accurate quantification over several logs of template
• Sensitive detection of up to 5 targets in 1 tube

The QuantiNova SYBR Green and Probe RT-PCR Kits can be used for gene expression analysis of RNA targets on any real-time cycler. This includes the Rotor-Gene Q and instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche and Agilent.

The QuantiNova Multiplex RT-PCR Kit can be used for multiplex gene expression analysis of RNA targets on any real-time cycler. To fully benefit from multiplexing, we recommend using an instrument that provides up to 5-plex capacity, such as the Rotor-Gene Q.