QIAseq Stranded mRNA Enrichment Kit (24)，1105688，Qiagen，凯杰

QIAseq UPXome RNA Library Kits enable single-day, high-throughput next-generation sequencing (NGS) RNA-seq library construction for use with Illumina NGS instruments. These RNA library kits feature ultraplex (UPX) pooling of cDNA and support complete transcriptome or 3’ RNA-seq starting with total RNA or enriched mRNA. When used with the QIAseq UX Index IL UDI Index Kits (sold separately), up to 18,432 samples can be ultraplexed together on a single flow cell. The complete solution includes online access to the RNA-seq analysis portal (for supported species), which allows researchers to start with FASTQ files and perform read alignment, differential gene expression and pathway analysis. Furthermore, qPCR and digital PCR assays for biological verification can be easily identified in QIAGEN GeneGlobe following data analysis. QIAseq UPXome RNA Library Kits are also supported with on-site software through QIAGEN’s CLC Genomics Workbench (sold separately).

• Start with 500 pg to 100 ng of total RNA or enriched mRNA
• Integrated QIAseq FastSelect RNA removal reagent suppresses mitochondrial and cytoplasmic ribosomal RNA or other unwanted RNAs
• Includes protocols for library construction for complete transcriptome or 3’ RNA-seq starting with total RNA or enriched mRNA
• Ultraplex pooling of 8 to 24 cDNA reactions increases sample throughput while reducing waste and consumables
• Includes access to the RNA-seq Analysis Portal for supported species

QIAseq UPXome RNA Library Kits support library construction for transcript identification, fusion gene discover and high-throughput gene expression. Input RNA can be high-quality or fragmented, allowing for RNA-seq libraries from tissues, exosomes, FFPE samples and cell lines. RNA from eukaryotic, prokaryotic and fungi samples is also supported.