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General description
N-terminal 6×His-tagged Drosophila NAP1 expressed in E. coli.
Nucleosome assembly proteins (NAP) are involved in moving histones into the nucleus, nucleosome assembly and chromatin fluidity. They affect the transcription of many genes.
Application
Lot # R0803D0002: 12.5 µg NAP1 has been successfully used for 1 chromatin assembly reaction with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).
Physical form
10 mM K-HEPES pH 7.6, 10 mM KCl, 50 mM NaCl, 0.1 mM EDTA, 10%(v/v) glycerol, 0.01% NP-40, 0.2 mM PMSF, 1mM DTT. Frozen solution.
Format: Purified
Preparation Note
Stable for 6 months at -80°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Analysis Note
Activity is routinely tested with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).
Other Notes
Specific Activity: Varies with lot
Varies with individual lot. Lot R0803D0002 has purity 90% by SDS-PAGE and Coomassie blue staining
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
| Quality Segment | 100 |
| mol wt | Mw 43 kDa |
| manufacturer/tradename | Upstate® |
| UniProt accession no. | Q9W1G7 |
| shipped in | dry ice |
| Gene Information | Drosophila ... NAP1(37798) |
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