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General description
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure; however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
~ 200 kDa
Immunogen
Epitope: Unknown
Purified neurofilament polypeptides (Debus et al., 1983).
Application
Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated is an antibody against Neurofilament H for use in IH & IC.
Immunohistochemistry:
5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Biochem/physiol Actions
Reactivity with other species has not been tested.
This antibody recognizes Neurofilament H.
Physical form
Protein G Purified
Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.1% sodium azide.
Preparation Note
Stable for 6 months at 2-8ºC from date of receipt.
Analysis Note
Control
Rat Cortex Cells.
Immunocytochemical Analysis: 1:250 dilution of this lot detected Neurofilament-H in rat cortex cell culture.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | NE14, monoclonal |
| species reactivity | human, rat, pig, porcine |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_066554.2 |
| UniProt accession no. | P12036 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NEFH(4744) pig ... Nefh(100156492) rat ... Nefh(24587) |
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