- 商品介绍
- 规格参数
- 包装参数
Immunogen
GS-NEM conjugated to KLH
Application
Detect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.
Immunohistochemistry: 1:100 (paraformaldehyde fixation)
Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.
ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.
Flow cytometry: 1:100 - 1:1,000
Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 μM NEM (N-ethyl maleimide) for 30 min at RT.
Optimal working dilutions must be determined by end user.
Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
Biochem/physiol Actions
Reactive with thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins. MAB3194 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow cytometry, sample must first be reacted with NEM.
Physical form
Format: Purified
Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 8.1GSH, monoclonal |
| species reactivity (predicted by homology) | mammals |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
¥ 4164.19
-
¥ 2814.26
-
¥ 1683.78
-
¥ 1259.40
-
¥ 4173.75
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 3290.05
-
询价
-
无菌，PETG 方形有刻度培养基瓶，带盖，聚对苯二酸乙二醇酯共聚物，天然高密度聚乙烯盖，500毫升容量，每箱40，40/箱，342020-0500，Nalgene，Thermofisher，赛默飞世尔¥ 3684.34
-
¥ 2268.97
-
¥ 1749.15
-
¥ 2277.64
-
¥ 2132.12
微信小程序
7X24小时在线咨询