- 商品介绍
- 规格参数
- 包装参数
Immunogen
HSP70 from HeLa cells.
Application
Immunoblotting: 0.5-1 μg/mL
Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)
Flow cytometry
Optimal working dilutions must be determined by end user.
This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.
Biochem/physiol Actions
Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.
POSITIVE CONTROL:
HeLa cells, BT474 cells or breast carcinoma.
Physical form
Format: Purified
Analysis Note
Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | W27, monoclonal |
| species reactivity | bovine, human, monkey |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable (paraffin),immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2a |
| NCBI accession no. | NM_002154.3,NM_198431.1 |
| UniProt accession no. | P34932 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HSPA4(3308) |
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