Human Embryonic Germ Layer Characterization Kit,1KIT,SCR030,Sigma

销售价: ¥ 10254.00 / 件
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订货号 0BW1241
品牌型号 Sigma SCR030
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最小订货量 1件
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产品介绍 Product Description

General description

Human embryonic stem cells (HESC) are pluripotent stem cells that are capable of self-renewal and of differentiation in vitro and in vivo into a wide variety of cell types, including derivatives of all three embryonic germ layers. These primary germ layers will eventually give rise to all the tissues and organs of the body.



Mesoderm: bone, cartilage, muscle, blood and vascular system

Endoderm: gastrointestinal tract, respiratory tract and endocrine glands (including liver and pancreas)

Ectoderm: nervous system, skin



CHEMICON®′s Human Embryonic Germ Layer Characterization Kit (SCR030) allows researchers to assess the differentiation potential of their HESC to form derivatives of all three embryonic germ layers. Included in the kit are antibodies that are routinely used by stem cell researchers to identify the lineage fates of HESC following either spontaneous or directed differentiation.



Mesoderm: Smooth Muscle Actin (CBL171)

Cardiac Troponin I (MAB1691-50UG)



Endoderm: Alpha Fetoprotein (2004189)



Ectoderm: Nestin (ABD69)

MAP-2 (MAB3418-50UG)


Application

CHEMICON′s Human Embryonic Germ Layer Characterization Kit allows researchers to assess the differentiation potential of their Human Embryonic Stem Cell (HESC) to form derivatives of all three embryonic germ layers.
Research Category
Stem Cell Research


Preparation Note

Maintain components according to the individual data sheets. Antibodies to alpha fetoprotein (2004189) and nestin (ABD69) are to be stored at -20°C (SCR030 - Part 1). Antibodies to smooth muscle actin (CBL171), cardiac troponin I (MAB1691-50UG) and MAP-2 (MAB3418-50UG) are to be stored at 2 - 8°C (SCR030 - Part 2).


Other Notes

50 μL of Alpha Fetoprotein mouse monoclonal 2004189

50 μg of Smooth Muscle Actin mouse monoclonal CBL171

50 μL of Nestin rabbit polyclonal ABD69

50 μg of Cardiac Troponin I mouse monoclonal MAB1691-50UG

50 μg of MAP-2 mouse monoclonal MAB3418-50UG


Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
Quality Segment100
species reactivityhuman
manufacturer/tradenameChemicon®
technique(s)cell culture | stem cell: suitable,immunocytochemistry: suitable
inputsample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
shipped inwet ice
storage temp.-10 to -25°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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