- 商品介绍
- 规格参数
- 包装参数
General description
Transgelin, also known as 22 kDa actin-binding protein, Protein WS3-10, and Smooth muscle protein 22-alpha (SM22-alpha), is an actin cross-linking protein that is involved in cellular calcium interactions and contractile properties of the cytoskeleton contributing to replication and senescence in smooth muscle tissue. Transgelin is overexpressed in senescent human fibroblasts.
~22 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human Transgelin/SM22a.
Application
Anti-Transgelin/SM22a Antibody, clone 20F2.2 is a highly specific mouse monoclonal antibody, that targets Transgelin & has been tested in western blotting & ICC.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western Blotting Analysis: 1 µg/ml from a representative lot detected Transgelin/SM22a in 10 µg of NIH/3T3 and PC12 cell lysates.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Transgelin/SM22a in NIH/3T3 cells.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
U20S cell lysate
Evaluated by Western Blotting in U20S cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Transgelin/SM22a in 10 µg of U20S cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 20F2.2, monoclonal |
| species reactivity | human, mouse, rat |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001001522 |
| UniProt accession no. | Q01995 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TAGLN(6876) |
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