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General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain. Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions. Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
A linear peptide corresponding to human Histone H3 monomethylated at Lys79.
Application
Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
This Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.
Biochem/physiol Actions
This antibody reacts to Histone H3 monomethylated at Ly79 (K79me1). No cross reactivity with dimethylated Lys79 (K79me2) or trimethylated
Lys79 (K79me3), or other methylations in histone H3.
Physical form
Format: Purified
Protein A purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Evaluated by Western Blotting in HeLa acid extract and HeLa cell lysate.
Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys79) in HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | RM147, monoclonal |
| species reactivity | human |
| technique(s) | multiplexing: suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | P84243 |
| shipped in | wet ice |
| target post-translational modification | monomethylation (Lys79) |
| Gene Information | human ... H3F3B(3021) |
| 长度(mm) | |
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