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- 规格参数
- 包装参数
General description
62 kDa
The activity of the main glutamate transporter in the CNS, Excitatory amino acid transporter 2 (also known as GLT1), can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the excitatory amino acid transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the excitatory amino acid transporter 2. Exposure to PMA increases the ubiquitylation of the excitatory amino acid transporter 2 in transfected cells and in the rat brain, and this ubiquitylated excitatory amino acid transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Excitatory amino acid transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery.
Immunogen
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of human Excitatory amino acid transporter 2.
Application
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience
This Anti-Excitatory amino acid transporter 2 Antibody, clone G6 is validated for use in WB, IH, IC for the detection of Excitatory amino acid transporter 2.
Western Blot Analysis: 0.5 µg/ml from a previous lot detected Excitatory amino acid transporter 2 on 10 µg of human brain tissue lysate.
Biochem/physiol Actions
This antibody recognizes the C-terminus of Excitatory amino acid transporter 2.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Excitatory amino acid transporter 2 on 10 µg of mouse brain tissue lysate.
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | G6, monoclonal |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat (100% immunogen homology) |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_004162 |
| UniProt accession no. | P43004 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SLC1A2(6506) |
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¥ 3109.49
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¥ 3290.05
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