- 商品介绍
- 规格参数
- 包装参数
General description
Mitoferrin-1 (UniProt Q920G8; also known as mitochondrial iron transporter 1, mitochondrial solute carrier protein, solute carrier family 25 member 37) is encoded by the Slc25a37 (also known as Mfrn, Mfrn1, Mscp) gene (Gene ID 67712) in murine species. Mitoferrin-1 and mitoferrin-2 (Slc25a28, Mfrn2) mediate iron import into mitochondria. Mitoferrin-2 is expressed ubiquitously, whereas mitoferrin-1 is specifically induced in differentiating erythroid cells. Mfrn1 knockout in zebrafish and mice disrupts heme and [Fe-S] cluster synthesis as a result of a severe reduction in mitochondrial iron in erythroid progenitors.
~34 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of mouse Mitoferrin-1/Mfrn1.
Application
Research Category
Signaling
Research Sub Category
Developmental Signaling
This Anti-Mitoferrin-1/Mfrn1 Antibody is validated for use in Western Blotting for the detection of Mitoferrin-1/Mfrn1.
Western Blotting Analysis: A 1:2,000 dilution of this antiserum detected mitoferrin-1 upregulation in mitochondria lysates from mouse erythroleukemia (MEL) cultures chemically induced to undergo erythroid maturation (Courtesy of Dr Barry H. Paw, Brigham and Women′s Hospital, Boston, MA).
Western Blotting Analysis: A representative lot detected reduced mitoferrin-1 expression due to heterozygous gene trap insertion in the Mfrn1 gene (Mfrn1+/gt) and loss of mitoferrin-1 expression due to homozygous Mfrn1 gene insertion (Mfrn1gt/gt) in mouse embryonic stem cells (mESCs) undergoing primitive erythropoiesis (Chung, J., et al.(2014). J Biol Chem. 289(11):7835-7843).
Western Blotting Analysis: A representative lot detected upregulated mitoferrin-1 expression following DMSO-induced erythroid maturation in mouse erythroleukemia (MEL) cultures (Shah, D.I., et al. (2012). Nature. 491(7425):608-612).
Biochem/physiol Actions
This antiserum does not react with spliced isofrms 2, 3, 4, and 5.
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in mouse embryonic stem (mES) cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected mitoferrin-1 in mouse embryonic stem (mES) cell lysates.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_080607 |
| UniProt accession no. | Q920G8 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Slc25A37(67712) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 1779.22
-
¥ 2068.25
-
询价
-
询价
-
询价
-
询价
-
¥ 3372.68
-
¥ 4404.38
-
¥ 4020.77
-
¥ 1783.21
-
¥ 4476.05
-
¥ 3662.39
-
¥ 5426.00
-
¥ 18741.69
-
询价
微信小程序
7X24小时在线咨询