c-MET, GST-Fusion, Human, Recombinant, S. frugiperda,10UG,325885-10UGCN,Sigma

销售价: ¥ 5659.76 / 件
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订货号 0BW0573
品牌型号 Sigma 325885-10UGCN
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Recombinant, human MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.


Packaging

Please refer to vial label for lot-specific concentration.


Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.


Preparation Note

Following initial thaw, aliquot and freeze (-70°C).


Other Notes

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)6:2:5:1 per min at 30°C, using variable concentrations of ATP (0.1-1.48 µM).
Palka, H.L., et al. 2003. J. Biol. Chem.278, 5278.
Vadnais, J., et al. 2002. J. Biol. Chem.277, 48342.
Crostella, L., et al. 2001. Oncogene20, 3735.
Guiton, M., et al. 1994. J. Biol. Chem269, 30370.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
recombinantexpressed in baculovirus infected Sf21 cells
Quality Segment100
formliquid
specific activity≥30 units/mg protein
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze,avoid repeated freeze/thaw cycles
shipped inwet ice
storage temp.−70°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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