Anti-phospho ULK1 Antibody (Thr574)，100UG，ABC212，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.
~120 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

KLH-conjugated linear peptide corresponding to mouse ULK1 phosphorylated at Thr574.

Application

Detect Autophagy-related protein;ATG1 using this rabbit polyclonal antibody, Anti-phospho ULK1 Antibody (Thr574) validated for use in western blotting & Peptide Inhibition Assay.
Western Blotting/Peptide Inhibition Analysis: 0.5 µg/mL from a representative detected phospho ULK1 in WB/PIA.

Analysis Note

Evaluated by Western Blotting in untreated and Calyculin A with okadaic acid treated Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho ULK1 (Thr574) in 10 µg of Jurkat cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

技术参数 Specifications

biological source	rabbit
Quality Segment	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)	inhibition assay: suitable (peptide),western blot: suitable
NCBI accession no.	NP_033495
UniProt accession no.	O70405
shipped in	wet ice
target post-translational modification	phosphorylation (pThr574)
Gene Information	human ... ULK1(8408)

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