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General description
Galectin-3 belongs to the galectin gene family defined by the specificity of a carbohydrate recognition domain (CRD) for Gal/Lac and expression is usually elevated in a wide range of neoplastic cell types. The galectin CRD consists of 135 amino acids. Galectin-3 has been shown to effect tumor development and progression.
~28 kDa observed
Immunogen
Plasma membrane glycoproteins corresponding to mouse Galectin-3.
Application
Anti-Galectin-3 Antibody, clone M3/38 is an antibody against Galectin-3 for use in WB, IP, IH & IC.
Western Blot Analysis: 1:50,000 dilution from a previous lot detected Galectin-3 in 10 µg of NIH/3T3 cell lysate.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Springer, T., et al. (1981). THE JOURNAL OF BIOLOGICACHLE MISTRY. 256(8):3833-3839.)
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Gasbarri, A., et al. (1999). Journal of Clinical Oncology. 17(11):3494-3502.)
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Gasbarri, A., et al. (1999). Journal of Clinical Oncology. 17(11):3494-3502.)
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1:50,000 dilution of this antibody detected Galectin-3 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rat |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | M3/38, monoclonal |
| species reactivity | mouse, human |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001139425 |
| UniProt accession no. | P16110 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... LGALS3(3958) |
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