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General description
RasGRP1, also known as Calcium and DAG-regulated guanine nucleotide exchange factor II (CalDAG-GEFII) or Ras guanyl-releasing protein (RASGRP), and encoded by the gene RASGRP1/RASGRP, functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. RasGRP1 activates the Erk/MAP kinase cascade and couples T-lymphocytes and B-lymphocytes antigen receptors to the activation of Ras. Hence, it regulates T-cells and B-cells development, homeostasis and differentiation. Additionally, RasGRP1 functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating allergic responses. RasGRP1 may also function in other cell types’ differentiation. RasGRP1 is localized to the cytoplasm and cell membrane; it is a peripheral membrane protein in the Golgi/ER membranes. In neurons, RasGRP1 localizes to somata and dendrites but not to axons of hippocampal pyramidal cells of in the brain and in particular the cerebellum, cortex and amygdata. RasGRP1 also is expressed in the hematopoietic system including T cells and RasGRP1 is also found expressed developmentally in fetal brain and kidney tissues. Defects in RasGRP1 may be associated with Systemic lupus erythematosus (SLE) a chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Interestingly it is thought that aberrantly spliced isoforms or other mutations of RASGRP1 give rise to dysregulation of various signaling processes which in turn contribute to the development of autoimmunity in SLE patients.
~90 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing at ~62 kDa, ~55 kDa, and ~68 kDa
Immunogen
GST-tagged recombinant protein corresponding to human RasGRP1.
Application
Detect RasGRP1 using this mouse monoclonal antibody, Anti-RasGRP1 Antibody & clone 10.1 validated for use in western blotting.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected RasGRP1 in 10 µg of human cerebellum tissue lysate.
Flow Cytometry Analysis: A representative lot detected RasGRP1 in malignant lymphoid cells treated with tipifarmib (Ding, H., et al. (2011). Blood. 118:4872-4881).
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in MOLT-4 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected RasGRP1 in 10 µg of MOLT-4 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 10.1, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001122074 |
| UniProt accession no. | O95267 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RASGRP1(10125) |
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