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General description
Acetylation of lysine residues in proteins has emerged as an important mechanism in regulation and coordination of normal and pathophysiological cell metabolism. Lysine HMGylation is a newer post-translational protein modification that occurs under normal basal conditions. In murine models of HMG-CoA lyase deficiency, higher levels of protein lysine HMGylation has been observed. This rabbit polyclonal antibody specifically detects chemically modified proteins containing hydroxymethylglutaryl-lysine modifications, but not unmodified proteins. (Ref.: Wagner, GR et al. (2017). Cell Metabolism 25(4); 823-837).
~75 kDa observed (BSA conjugate). Uncharacterized bands may be observed in some lysate(s).
Immunogen
3-hydroxy-3-methyl-glutaryl (HMG)-Lysine conjugated to BSA.
Epitope: unknown
Application
Anti-HMG-Lysine, Cat. No. ABS2108, is a rabbit polyclonal antibody that detects hydroxymethylglutaryl-lysine conjugated proteins and has been tested for use in Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A representative lot detected HMG-Lysine in Western Blotting applications (Wagner, G.R., et. al. (2017). Cell Metab. 25(4):823-837; Anderson, K.A., et. al. (2017). Cell Metab. 25(4):838-855.e15).
Western Blotting Analysis: A 1:500 dilution from a representative lot detected HMG-Lysine in HMG-BSA conjugate (Courtesy of Dr Kristin Anderson from Duke University).
Biochem/physiol Actions
This rabbit polyclonal antibody detects BSA-conjugated 3-hydroxy-3-methyl-glutaryl (HMG)-Lysine. It recognizes BSA chemically modified to contain HMG-lysine modifications, but not unmodified BSA.
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in PBS with 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting with HMG-BSA conjugate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected HMG-Lysine in HMG-Lysine-BSA conjugate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity (predicted by homology) | all |
| packaging | antibody small pack of 25 μL |
| technique(s) | western blot: suitable |
| isotype | IgG |
| target post-translational modification | unmodified |
| 长度(mm) | |
| 宽度(mm) | |
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