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General description
Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
~160 kDa observed. The calculated molecular weight is 137 kDa, however IRS-2 has been shown as a ~160 kDa band in western blots (Rother, K., et al. (1998). Journal of Biological Chemistry. 273(28):17491–17497.)
Immunogen
Epitope: Phosphorylated Ser388
KLH-conjugated linear peptide corresponding to human IRS-2 phosphorylated Ser388.
Application
Anti-phospho-IRS-2 (Ser388) Antibody detects level of phospho-IRS-2 (Ser388) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay.
Peptide Inhibition Assay Analysis: 10 µg from a representative lot blocked IRS-2 on insulin treated HEK293 cells.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
Biochem/physiol Actions
This antibody recognizes IRS-2 phosphorylated at Ser388.
Physical form
Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Untreated and insulin treated HEK293 cell lysates
Evaluated by Western Blot in untreated and insulin treated HEK293 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | inhibition assay: suitable (peptide),western blot: suitable |
| NCBI accession no. | NP_003740 |
| UniProt accession no. | Q9Y4H2 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer388) |
| Gene Information | human ... IRS2(8660) |
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