- 商品介绍
- 规格参数
- 包装参数
Immunogen
A purified peptide of rat P2X4 protein (amino acids 370-388) (Accession P51577).
Application
Anti-P2X4 Receptor Antibody detects level of P2X4 Receptor & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1-1.5 μg/mL (1:200:1:300) using ECL
Immunohistochemistry on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes a full-length P2X4 purinergic receptor protein and not with other known proteins tested so far.
SPECIES REACTIVITIES: The immunogen sequence is identical in mouse. Other species have not been tested.
Physical form
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Preparation Note
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| NCBI accession no. | NM_002560.2 |
| UniProt accession no. | Q99571 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | rat ... P2Rx4(29659) |
| 长度(mm) | |
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| 重量(kg) |
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