Anti-Uncoupling Protein 1 Antibody，50UG，AB3036，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

Immunogen

A 12 aa peptide at the C-terminus of the mouse/rat UCP1 (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993).

Application

Detect Uncoupling Protein 1 using this Anti-Uncoupling Protein 1 Antibody validated for use in ELISA & WB.
Western Blotting: 1-10 μg/mL using Chemiluminescence technique. An antibody made to this epitope has detected a protein of ~32 kDa in brown adipose tissues (Feil & Rafael 1994).

ELISA: 1:10,000-1:100,000; using 50-100 ng UCP1 control peptide/well.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Uncoupling Protein 1 (UCP1), mouse. Mouse/rat UCP1 is a 307 amino acid mitochondrial uncoupling protein (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). It is only 59% homologous with UCP2 (Fleury et al. 1997). Human UCP1 is located on chromosome 4 (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). UCP1 is primarily expressed in brown adipose tissues.

The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to rat, 91% with golden hamster, 83% with rabbit and 75% with human and canine UCP1.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with less than 0.1% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

技术参数 Specifications

biological source	rabbit
Quality Segment	100
conjugate	unconjugated
antibody form	affinity purified immunoglobulin
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	mouse
manufacturer/tradename	Chemicon^®
technique(s)	ELISA: suitable,western blot: suitable
NCBI accession no.	NM_021833.3
UniProt accession no.	P25874
shipped in	dry ice
target post-translational modification	unmodified
Gene Information	mouse ... Ucp1(22227)

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