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General description
Ras association domain-containing protein 1 (UniProt: Q9NS23; RASSF1) is encoded by the RASSF1 (also known as RDA32) gene (Gene ID: 11186) in human. RASSF1 serves as a potential tumor suppressor and is shown to be essential for death receptor-dependent apoptosis. It mediates the activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. In association with MOAP1, it promotes Bax conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Eight isoforms of RASSF1 have been described that are produced by alternative promoter usage and alternative splicing. The immunogen sequence used to generate this antibody is conserved in isoforms A, D, E, F and G. Isoform A is ubiquitously expressed and is shown to localize to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Isoform A is reported to interact with CDC20, an activator of the anaphase-promoting complex (APC), resulting in the inhibition of APC activity and mitotic progression. It Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. It also disrupts interactions among MDM2, DAXX and USP7 and thereby contributes to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
~39 kDa observed; 39.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Ras association domain-containing protein 1.
Application
Anti-RASSF1, Cat. No. ABS1484, is a rabbit polyclonal antibody tht detects Ras association domain-containing protein 1 in human and rat tissues and has been tested for use in Flow Cytometry, Immunohistochemistry (paraffin), and Western Blotting.
Flow Cytometry Analysis: 1 µg from a representative lot detected RASSF1 in Jurkat cells.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RASSF1 in human ovarian cancer, human placenta, and human pancreas tissue sections.
Research Category
Signaling
Biochem/physiol Actions
This rabbit polyclonal antibody detects Ras association domain-containing protein 1 in human and rat tissues. It targets an epitope within 14 amino acids from the N-terminal region.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in rat liver tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected RASSF1 in 10 µg of rat liver tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, rat |
| packaging | antibody small pack of 25 μL |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_733832.1 |
| UniProt accession no. | Q9NS23 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... RASSF1(11186) |
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