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General description
Cytochrome b (UniProt: P00156; also known as Complex III subunit 3, Complex III subunit III, Cytochrome b-c1 complex subunit 3, Ubiquinol-cytochrome-c reductase complex cytochrome b subunit) is encoded by the MT-CYB (also known as COB, CYTB, MTCYB) gene (Gene ID: 4519) in human. Cytochrome b is a mitochondrial inner membrane protein that is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex). It is a part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c and contributes to the generation of a proton gradient across the mitochondrial membrane, which is used for ATP synthesis. The MT-CYB gene is a good phylogenetic marker. Defects in MT-CYB gene are a rare cause of mitochondrial dysfunction underlying different myopathies, which include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy, and sporadic mitochondrial myopathy. Some cases of Leber hereditary optic neuropathy (LHON) are also linked to defects in MT-CYB gene, which result in acute or subacute loss of central vision, due to optic nerve dysfunction.
~28 kDa observed; 42.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: N-terminus
KLH-conjugated Synthetic peptide corresponding to the N-terminus of human cytochrome b.
Application
Research Category
Signaling
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Cytochrome b in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA)
Anti-Cytochrome b, clone 5B3-6E3, Cat. No. MABS2036, is a mouse monoclonal antibody that detects Complex III Cytochrome b and has been tested for use in Western Blotting.
Biochem/physiol Actions
Clone 5B3-6E3 detects cytochrome b in human mitochondria. It targets an epitope within the N-terminal region.
Physical form
Format: Purified
Protein L
Purified mouse monoclonal antibody IgG2b in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Cytochrome b in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 5B3-6E3, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | YP_003024038.1 |
| UniProt accession no. | P00156 |
| target post-translational modification | unmodified |
| Gene Information | human ... MT-CYB(4519) |
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