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General description
Major intrinsically disordered NOTCH2-binding receptor 1-like (UniProt: P59773; also known as Membrane integral NOTCH2-associated receptor 2) is encoded by the MINAR2 (also known as KIAA1024L) gene (Gene ID: 100127206) in human. MINAR2, a highly conserved, intrinsically disordered member of the MINAR family of proteins, has an extracellular domain, a single transmembrane domain, and a few amino acids in its cytoplasmic tail. Its expression is observed in the brain with high levels detected in midbrain, hypothalamus, and the pituitary gland. Loss of MINAR2 is reported to cause motor dysfunctions in murine models that resemble Parkinson′s disease. MINAR2 knockout mice display significant alterations in their neuronal function, loss of TH positive neurons in pars compacta, and higher accumulation of -synuclein protein. It is reported to be localized to endoplasmic reticulum (ER) compartments and interacts with NOTCH2. ER stress, NOTCH folding, and its trafficking from the ER have been proposed to play a role in motor deficits associated with progression of Parkinson s disease. MINAR2 has also been linked to progressive hearing loss with alteration sin the stereocilia of hair cells. Loss of MINAR2 is shown to reduce cholesterol localization to the hair bundles in the ear that aggravates hair cell deficits. (Ref.: Bademci, G., et al. (2022) Proc. Natl. Acad. Sci. USA. 119(26); e2204084119; Ho, RX-Y., et al. (2020). Brain Commun.2(1); fcaa047).
Immunogen
A linear peptide corresponding to 14 amino acids from the C-terminal half of human Membrane integral NOTCH2-associated receptor 2 (MINAR2).
Application
Quality Control Testing
Evaluated by Western Blotting in lysates from HEK293 cells transfected mouse MINAR2.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected mouse MINAR2 in lysates from HEK293 cells transfected mouse MINAR2, but not in lysates from untransfected cells.
Tested Applications
Immunohistochemistry Applications: A representative lot detected MINAR2/KIAA1024L in Immunohistochemistry application (Ho, R., et. al. (2020). Brain Commun. 2(1):fcaa047).
Immunocytochemistry Analysis: A representative lot detected MINAR2/KIAA1024L in Immunocytochemistry applications (Ho, R., et. al. (2020). Brain Commun. 2(1):fcaa047).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
This rabbit polyclonal antibody detects Membrane integral NOTCH2-associated receptor 2 (MINAR2). It targets an epitope within 14 amino acids from the C-terminal half.
Physical form
Purified rabbit polyclonal antibody in PBS without azide.
Preparation Note
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| mol wt | calculated mol wt 21.57 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | horse, bovine, monkey |
| packaging | antibody small pack of 100 μg |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_001244237.1 |
| UniProt accession no. | P59773 |
| storage temp. | -10 to -25°C |
| target post-translational modification | unmodified |
| Gene Information | human ... MINAR2(100127206) |
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