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General description
Suppressor of fused homolog (UniProt: Q9UMX1; also known as SUFUH) is encoded by the SUFU (also known as UNQ650/PRO1280) gene (Gene ID: 51684) gene in human. SUFUH acts as a negative regulator in the hedgehog signaling pathway that is ubiquitously distributed in adult tissues. Three isoforms of SUFUH have been described that are produced by alternative splicing. Isoform 1 is detected in fetal brain, lung, kidney and testis and isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney. SUFUH is shown to down-regulate Gli1-and Gli2-mediated transactivation of target genes. Binding of SUFUH sequesters Gli proteins in the cytoplasm. It is a part of a corepressor complex that acts on DNA-bound Gli1 and may link Gli1 to beta-transducin repeat containing E3 Ubiquitin Protein Ligase (BTRC) and facilitate its proteasomal degradation. SUFUH regulates the formation of either the repressor form (Gli3R) or the activator form (Gli3A) of the full length form of GLi3 (Gli3FL). When complexed with SUFUH, Gli3FL remains in the cytoplasm and is maintained in a neutral state. Mutations in SUFU gene are known to cause medulloblastoma.
~54 kDa observed; 53.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST/His-tagged recombinant fragment corresponding to 177 amino acids from the C-terminal half of human Suppressor of fused homolog (SUFU).
Application
Anti-SUFU, clone 15G3.1, Cat. No. MABS1883, is a mouse monoclonal antibdoy that detects Suppressor of fused homolog (SUFU) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SUFU in human cerebellum, human testis, human pancreas, and human skin tissues.
Research Category
Signaling
Biochem/physiol Actions
Clone 15G3.1 detects Suppressor of fused homolog (SUFU) in human and murine cells. It targets an epitope within 177 amino acids from the C-terminal half.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in NIH-3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SUFU in NIH-3T3 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 15G3.1, monoclonal |
| species reactivity | mouse, human |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_057253.2 |
| UniProt accession no. | Q9UMX1 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... SUFU(51684) |
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