Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1，100UG，MABT379，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Discovered on gastrointestinal stromal tumors protein 1 (Dog1), also known as Anoctamin-1/TMEM16A, is a novel multi-pass membrane protein belonging to the anoctamin family. Anoctamin-1/TMEM16A functions as a Ca2+-activated Cl- channel and is a required component of tracheal development. Expression of Anoctamin-1/TMEM16A is elevated in the gastrointestinal stromal tumors (GISTs), KIT signaling-driven mesenchymal tumors of the GI tract. It is also expressed at more moderate levels in epithelia from foregut, kidney, lung, pancreas and salivary glands. Recent studies suggest that it may serve a roll as a diagnostic marker in the classification of GISTs.
~114 kDa and ~98 kDa observed. Three isoforms are known to exist at 114 kDa (isoform 1), 98 kDa (isoform 2), and 74 kDa (isoform 3).

Immunogen

Epitope: N-terminal cytoplasmic domain
GST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human Anoctamin-1/TMEM16A.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Anoctamin-1/TMEM16A in human prostate cancer sections.
This Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1 is validated for use in western blotting & IHC for the detection of Anoctamin-1/TMEM16A.

Biochem/physiol Actions

This antibody recognizes isoforms 1 and 2 of Anoctamin-1 based upon immunogen design.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in PC-3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Anoctamin-1/TMEM16A in 10 µg of PC-3 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

技术参数 Specifications

biological source	mouse
Quality Segment	100
conjugate	unconjugated
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	5F8.1, monoclonal
species reactivity	human
technique(s)	immunohistochemistry: suitable,western blot: suitable
isotype	IgG1κ
NCBI accession no.	NP_060513
UniProt accession no.	Q5XXA6
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	human ... ANO1(55107)

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