Anti-ARF1 Antibody, clone 3F1,100UG,05-1427,Sigma

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订货号 0BV8461
品牌型号 Sigma 05-1427
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.
~21 kDa


Immunogen

Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.


Application

Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer


Biochem/physiol Actions

Recognizes human ARF1


Physical form

Format: Purified
Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide


Analysis Note

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.


Other Notes

Replaces: MAB3779


技术参数 Specifications
biological sourcemouse
Quality Segment100
conjugateunconjugated
antibody formpurified immunoglobulin
antibody product typeprimary antibodies
clone3F1, monoclonal
species reactivityrat, human, mouse
technique(s)ELISA: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable
isotypeIgG2a
NCBI accession no.NM_001024226.1
UniProt accession no.P84077
shipped inwet ice
target post-translational modificationunmodified
Gene Informationhuman ... ARF1(375)
mouse ... Arf1(11840)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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