ChemiSCREEN Membrane Preparation Recombinant Human MC ₁ Melanocortin Receptor，200UNITS，HTS156M，Sigma

General description

The melanocortin-1 receptor (MC₁) is a member of the G-protein-coupled receptor family of proteins that is responsible for regulating skin and hair color. The MC₁ receptor is regulated by the physiological agonists alpha-melanocyte-stimulating hormone (alphaMSH) and adrenocorticotropic hormone (ACTH), and antagonist agouti signaling protein (ASP) (Rouzud et al., 2005). In 1995 a landmark study demonstrated that over 80% of humans with red hair or fair skin have a dysfunctional variant of the MC₁ gene (Valverde et al., 1995). MC₁ is, therefore, a major determinant of sun sensitivity and genetic risk factor for melanoma and non-melanoma skin cancer. Millipore′s MC₁ membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of MC₁. The membrane preparations exhibit a Kd of 1.3nM for [¹²⁵I]-[Nle⁴, D-Phe⁷]-α-MSH (NDP-αMSH). With 0.3 nM [¹²⁵I]-NDP-αMSH, 5μg/well MC₁ Membrane Prep typically yields greater than 50-fold signal-to-background ratio.

Application

Human MC1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Radioligand binding assay and GTPγS binding.

Biochem/physiol Actions

• GPCR Class: A
• Protein Target: MC1
• Target Sub-Family: Melanocortin

Physical form

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C.

Preparation Note

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial