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General description
ATG3 is thought to be an autophagy-related ubiquitin carrier protein (E2)-like enzyme that is the go between for the conjugation on ATG8 and phosphatidylethanolamine (PE). This compound is necessary for autophagy, which is a process in which a cell is degraded by its own lysosomal process.
~37 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human ATG3.
Application
Anti-ATG3 Antibody detects level of ATG3 & has been published & validated for use in WB.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Biochem/physiol Actions
Other homologies: Ape, rhesus macaque, equine, porcine, and bovine (99% sequence homology). Mouse (98% sequence homology). Rat (97% sequence homology).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HL-60 cell lysate
Evaluated by Western Blot in HL-60 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected ATG3 in 10 µg of HL-60 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_071933 |
| UniProt accession no. | Q9NT62 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ATG3(64422) |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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