- 商品介绍
- 规格参数
- 包装参数
General description
110 kDa
Phosphoinositol-3-kinases (PI3 Kinase, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states. PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain. The class I PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain. There are 4 isoforms of the class I p110 catalytic domains. Class I(A) include α, β, γ and class I(B). They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors. Once activated, PI3K generates phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2. It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus de-activating the effects of PI3K. Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release. One of the primary roles of PI3K is its regulation of cell growth and cell metabolism. Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K. PI3 kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/ inflammatory disorders.
Immunogen
Epitope: Internal
KLH-conjugated synthetic linear peptide corresponding to an internal region of class I catalytic domain of PI3 Kinase that is specific to the p110 alpha isoform.
Application
Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110α validated for use in WB, IP, IH, ELISA.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Biochem/physiol Actions
Recognizes PI3 Kinase p110α.
Physical form
Immunogen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
Preparation Note
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Evaluated by western blot on Jurkat cell extract.
Western Blotting Analysis: 0.5 µg/mL (1:2,000) dilution of this antibody detected PI3K, p110α in Jurkat cell extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-399
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, rat |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_006209.2 |
| UniProt accession no. | P42336 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PIK3CA(5290) rat ... Pik3Ca(170911) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
¥ 4164.19
-
询价
-
¥ 2814.26
-
询价
-
询价
-
¥ 1724.25
-
¥ 3442.25
-
¥ 9737.84
-
询价
-
询价
-
询价
-
¥ 3290.05
-
询价
-
¥ 20380.83
-
¥ 2882.02
-
¥ 2307.00
-
¥ 3404.94
-
¥ 2406.69
-
¥ 10685.48
微信小程序
7X24小时在线咨询