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General description
Apoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.
~141 kDa observed; 141.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
A recombinant fragment corresponding to the first 97 amino acids of mouse Apaf-1.
Epitope: N-terminus
Application
Anti-Apaf-1, clone 13F11, is a rat monoclonal antibody that detects Apaf-1 in mouse and rat. It has been tested for use in Western Blotting.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: 1 ug/mL from a representative lot detected Apaf-1 in mouse bone marrow tissue lysate.
Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Vaughn, A.E., et. al. (2007). Cell Death Differ. 14(5):973-81; Wright, K.M., et. al. (2004). J Cell Biol. 167(2):303-13).
Biochem/physiol Actions
Clone 13F11 specifically detects Apaf-1 in mouse and rat. It targets an epitope within the first 97 amino acids in the N-terminal region.
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse lymph node tissue lysate.
Western Blotting Analysis: 1 ug/mL of this antibody detected Apaf-1 in mouse lymph node tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: MAB3504
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 13F11, monoclonal |
| species reactivity | rat, mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001036023 |
| UniProt accession no. | O88879 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Apaf1(11783) |
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmPES滤膜，孔径0.45um,适合瓶颈尺寸33mm，12/箱，295-3345，Nalgene，Thermofisher，赛默飞世尔¥ 1938.54
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