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General description
Dipeptidyl peptidase 4 (EC 3.4.14.5; UniProt P27487; also known as ADABP, ADCP-2, Adenosine deaminase complexing protein 2, DPP IV, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase 4, Dipeptidylpeptidase IV, TP103) is encoded by the DPP4 (also known as CD26, ADCP2) gene (Gene ID 1803) in human. DPPIV is a homodimeric serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. It is involved in many cellular processes such as activation of cytokines, differentiation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. Also, DPPIV acts as a functional receptor for the newly discovered Middle East respiratory syndrome (MERS) coronavirus.
~100 kDa observed. Due to glycosylation, target band appears larger than the calculated molecular weight of 88.3 (MDPP) & 84.4 (SDPP) kDa Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: extracellular domain
GST-tagged recombinant protein corresponding to the extracellular domain of human DDPIV/CD26.
Application
Research Category
Stem Cell Research
Research Sub Category
Endothelial Stem Cells
This Anti-DDPIV/CD26 Antibody, clone 9F1.2 is validated for use in Western Blotting for the detection of DDPIV/CD26.
Biochem/physiol Actions
Reacts with both membrane-bound (MDPP) and soluble (SDPP) forms.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected DDPIV/CD26 in 10 µg of PC3 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 9F1.2, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001926 |
| UniProt accession no. | P27487 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... DPP4(1803) |
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