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General description
Dual specificity mitogen-activated protein kinase kinase 7 (EC 2.7.12.2; UniProt O14733; also known as c-Jun N-terminal kinase kinase 2, MAP kinase kinase 7, MAPK/ERK kinase 7, MAPKK 7, MEK 7, JNK-activating kinase 2, JNK kinase 2, JNKK 2, SAPK kinase 4, SAPKK-4, SAPKK4, Stress-activated protein kinase kinase 4) is encoded by the MAP2K7 (also known as JNKK2, MEK7, MKK7, PRKMK7, SKK4) gene (Gene ID 5609) in human. MKK7 belongs to the family of mitogen-activated protein kinase kinases (MAPKKs) involved in mediating cellular signal transduction in response to proinflammatory cytokines stimulation and environmental stresses. MKK7 and the related MKK4 function as crucial upstream transducers in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway by phosphorylating different JNK isoforms. MKK7 is activated by upstream MKKKs, whereby the MKKKs interact with MKK7 through its C-terminal DVD domain (a.a. 377-400) and activate MKK7 by phosphorylating serine and threonine residues at a S-K-A-K-T motif located in the MKK7 kinase domain (a.a. 120-380). Upon activation, MKK4 and MKK7 in turn phosphorylate specific tyrosine and threonine residues of the conserved T-P-Y motif in the activation loop of substrate JNK proteins. The N-terminal D domain (a.a. 37-57), consisting of a cluster of positively charged amino acids followed by alternating hydrophobic amino acids, is required for the recruitment of MAPKK substrates, such as JNK. Alternative splicing results in four isoforms. Isoform B (beta), gamma, and isoform 4 differ from the canonical isoform A (alpha) by a 43-a.a. expansion at a.a. 111, a 16-a.a. expansion at a.a. 42, and a 7-a.a. expansion at a.a. 312, respectively, of the MKK7alpha sequence.
~50 kDa observed. 47.35/51.75/48.94/48.05 kDa (human isoform 1/2/3/4, Met1 removed), 59.18/47.43/42.59/39.25/44.08/49.02/52.37/50.78 kDa (mouse isoform 1/2/3/4/5/6/7/8, Met1 removed) calculated.
Immunogen
Epitope: Internal (N-terminal half).
GST-tagged recombinant human MKK7/SKK4 internal fragment.
Application
Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-MKK7 Antibody, clone 3F6.5 is validated for use in Western Blotting, Immunocytochemistry for the detection of SKK4.
Western Blotting Analysis: 1 µg/mL from a representative lot detected MKK7/SKK4 in 10 µg of NIH/3T3 cell lysate.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected MKK7/SKK4 in HeLa, HepG2, and NIH/3T3 cells.
Biochem/physiol Actions
Clone 3F6.5 was raised against recombinant N-terminal fragment derived from human MKK7/SKK4 spliced isoform 1 (isoform A or alpha) and isoform 4. The corresponding region in mouse isoform 2 & 8 (isoform alpha & beta 2) differ from the human sequence by only one amino acid (human A69 vs. mouse T69) Cross-reactivity toward other human and murine spliced isoforms is highly expected, but has not been confirmed.
Physical form
Format: Purified
Protein G purified.
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected MKK7/SKK4 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3F6.5, monoclonal |
| species reactivity | human, mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_660186 |
| UniProt accession no. | O14733 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MAP2K7(5609) |
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