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General description
Platelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA′, PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea and isoform Delta14-15 are only detected in lung tissue. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting ′detachment′ signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.
82.54 kDa calculated.
Immunogen
Epitope: extracellular domain
PECAM-1 purified from human platelets.
Application
Anti-CD31/PECAM-1.2, clone MBC 78.2, Cat. No. MABF2034, is a mouse monoclonal that detects PECAM-1 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD31/PECAM-1.2 in Jurkat cells.
Agonist or Inhibitor Analysis: A representative lot was used in Agonist or Inhibitor applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8; Reedguist, K.A., et. al. (2000). J Cell Biol. 148(6):1151-8).
Flow Cytometry Analysis: 1 µg from a representative lot detected CD31/PECAM-1.2 in THP-1 cells.
ELISA Analysis: A representative lot detected CD31/PECAM-1.2 in ELISA applications (Mei, H., et. al. (2014). J Biol Chem. 289(30):20836-44).
Flow Cytometry Analysis: A representative lot detected CD31/PECAM-1.2 in Flow Cytometry applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8).
Immunoprecipitation Analysis: A representative lot detected CD31/PECAM-1.3 in Immunoprecipitation applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27).
Western Blotting Analysis: A representative lot detected CD31/PECAM-1.3 in Western Blotting applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27).
Research Category
Inflammation & Immunology
Biochem/physiol Actions
Clone MBC 78.2 detects CD31/PECAM-1 in human cells. It targets an epitope within the IgG homology domain 6.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Immunocytochemistry in THP-1 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CD31/PECAM-1.2 in THP-1 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | MBC 78.2, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | ELISA: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000433 |
| UniProt accession no. | P16284 |
| target post-translational modification | unmodified |
| Gene Information | human ... PECAM1(5175) |
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