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General description
High affinity nerve growth factor receptor (UniProt: P04629; also known as EC:2.7.10.1, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, Trk-A, gp140trk, p140-TrkA) is encoded by the NTRK1 (also known as MTC, TRK, TRKA) gene (Gene ID: 4914) in human. Trk-A is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 33-423), a transmembrane domain (aa 424-439), and a cytoplasmic domain (aa 440-796). The extracellular domain of Trk-A is composed by five domains: a leucine-rich region, two cysteine-rich domains and two immunoglobulin (Ig)-like domains. Trk-A serves as a receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous system through regulation of proliferation, differentiation, and survival of sympathetic and nervous neurons. It serves as a high affinity receptor for NGF, which is its primary ligand. It can also bind and be activated by neurotrophin-3 (NTF3). However, NTF3 only supports axonal extension through NTRK1 but does not affect neuron survival. Upon dimeric NGF ligand-binding, it undergoes homodimerization, autophosphorylation. and activation. Clone MNAC13 recognizes Trk-A with high affinity and specificity and displays a neutralizing activity toward the NGF/TrkA interaction. (Ref.: Covaceuszach, S., et al. (2005). Proteins. 58(3); :717-727; Jing, S., et al. (1992). Neuron. 9(6); 1067-1079).
Immunogen
BALB/C 3T3-transfected cells expressing human Trk-A protein.
Application
Quality Control Testing
Evaluated by ELISA with recombinant Human TrkA.
ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant Human TrkA.
Tested applications
ELISA Analysis: A representative lot detected TrkA in ELISA applications (Covaceuszach, S., et al. (2005). Proteins. 58(3):717-27).
Neutralizing: A representative lot neutralized TrkA activity in Neutralizing applications (Covaceuszach, S., et al. (2005). Proteins. 58(3):717-27; Ugolini, G., et al. (2007). Proc Natl Acad Sci USA. 104(8):2985-90).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TrkA, clone MNAC13, Cat. No. MABN2308, is a mouse monoclonal antibody that detects TrkA and is tested for use in ELISA and Neutralizing.
Biochem/physiol Actions
Clone MNAC13 is a mouse monoclonal antibody that detects TrkA. It targets an epitope within 17 amino acids within the first Ig-like C2 type domain from the extracellular domain.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
Preparation Note
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | MNAC13, monoclonal |
| mol wt | calculated mol wt 87.5 kDa |
| purified by | using protein G |
| species reactivity | human |
| packaging | antibody small pack of 100 μg |
| technique(s) | ELISA: suitable,neutralization: suitable |
| isotype | IgG1κ |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_001007793 |
| UniProt accession no. | P04629 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NTRK1(4914) |
| 长度(mm) | |
| 宽度(mm) | |
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¥ 1573.49
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