Anti-phospho-PKCη (Thr655) Antibody,100UL,07-877,Sigma

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订货号 0BV6756
品牌型号 Sigma 07-877
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

~80 kDa


Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-PKCη (Thr655).


Application

Immunoblot Analysis: A 0.50 μg/mL dilution of this lot detected phospho-PKCη (Thr655) in RIPA lysate of PMA treated Jurkat cells.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
This Anti-phospho-PKCη (Thr655) Antibody is validated for use in WB for the detection of phospho-PKCη (Thr655).


Biochem/physiol Actions

Predicted to cross-react with Mouse and Rat (100% homologous) based on sequence homology.
Recognizes phosphorylated PKCη (Thr655). Not expected to cross-react with other PKC isoforms


Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide).
Affinity purified


Preparation Note

Stable for 2 years at -80°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.


Analysis Note

Routinely evaluated by immunoblot.


Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerabbit
Quality Segment100
antibody formaffinity isolated antibody
antibody product typeprimary antibodies
clonepolyclonal
purified byaffinity chromatography
species reactivityhuman
manufacturer/tradenameUpstate®
technique(s)western blot: suitable
isotypeIgG
NCBI accession no.NM_006255.3
UniProt accession no.P24723
shipped indry ice
target post-translational modificationphosphorylation (pThr655)
Gene Informationhuman ... PRKCH(5583)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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