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General description
Caspase-8 (UniProt: O89110; also known as EC: 3.4.22.61, CASP-8, CASP8, MCH5, FLICE, MACH) is encoded by the Casp8 gene (Gene ID: 12370) in murine species. Caspase-8 is a cysteine protease that plays a major role in the extrinsic apoptotic signaling pathway via death receptors. In its inactive form it associates with FADD by interactions between their DED motifs and within the FAS-FADD-Caspase 8 complex caspase 8 undergoes self-cleavage to convert to the active form. Activated caspase 8 is subsequently released to the cytosol and it cleaves downstream effector caspases 3, 4, 6, 7, 9 and 10. Caspase 8 is expressed in wide variety of tissues and its highest expression has been reported in spleen, thymus, lung, liver, and kidney. Low levels of expression are observed in heart, brain, testis, and skeletal muscle. Caspase-8 is produced with propetides (aa 1-218 and 377-387) that are cleaved under appropriate signals to produce a p20 (aa 219-376) and a p10 (aa 388-480) subunit. Active caspase 8 is a heterotetramer that consists of two p20 and two p10 subunits.
~55 kDa observed; 55.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Recombinant p20 subunit of Mouse Caspase-8.
Application
Anti-Caspase-8, clone 3B10, Cat. No. MABC1606, is a rat monoclonal antibody that detects Caspase-8 and has been tested for use in Flow Cytometry and Western Blotting.
Flow Cytometry Analysis: 1 µg from a representative lot detected Caspase-8 in one million mouse embryonic fibroblasts (MEF-1).
Research Category
Apoptosis & Cancer
Biochem/physiol Actions
This rat monoclonal antibody detects Caspase-8 in murine species. It targets an epitope within the p20 subunit.
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG1 in PBS with 0.02% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse embryonic fibroblast (MEF-1) cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Caspase-8 in mouse embryonic fibroblast (MEF-1) cell lysates.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3B10, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001073595 |
| UniProt accession no. | O89110 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Casp8(12370) |
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