GMBS，100MG，442630-100MGCN，Sigma

General description

Heterobifunctional reagent useful for preparation of conjugates such as secondary antibodies and β-galactosidase for EIA procedures or for immobilizing enzymes on solid supports.
N-γ-maleimidobutyryloxysuccinimide ester (GMBS) is an aliphatic crosslinker for immunotoxins (IT).(1) It is used for the generation of conjugated Poly-γ-D-glutamic acid.

Application

GMBS is suitable for use:

• as a hetero-bifunctional cross-linking reagent for the immobilization of neural adhesion molecule L1 onto silicon dioxide surface
• for the crosslinking of biochips with functionalized gold nanoparticles
• for coating salinized capillary tube prior to neutravidin treatment in biosensor sandwich immunoassay

Preparation Note

Reconstitute to 10 mg/ml in tetrahydrofuran or DMSO. Add 1 ml of this stock solution dropwise, with stirring, to 10 ml of 20 mM phosphate buffer.

Other Notes

Rusin, K.M., et al. 1992. Biosens. Bioelectron. 7, 367.
Kitagawa, T., et al. 1983. J. Biochem. 94, 1160.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)