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General description
120 kDa (de Bruin, 1995)
CD30 is a transmembrane cytokine receptor belonging to the tumor necrosis factor (TNF) receptor superfamily. Mature CD30 has a molecular mass of 120 kDa and is derived from a 90 kDa precursor protein (de Bruin, 1995). The extracellular domain of CD30 is homologous to that of TNF receptor superfamily members, whereas there is no homology in teh cytoplasmic domain, suggesting major differences in signaling mechanisms (Falini, 1995). CD30 expression is found on Hodgkin and Reed-Sternberg (H-RS) cells, and on activated B and T lymphocytes (de Bruin, 1995). The antibody from the Ber-H2 hybridoma cell line was included in the Fourth International Workshop on Human Leukocyte Differentiation Antigens and was found to belong to the CD30 cluster (Schwarting, 1989).
Immunogen
Co-cell line established from a human with Hodgkin′s disease of T-cell lineage.
Application
Milli-Mark Anti-CD30-FITC Antibody, clone Ber-H2 detects level of CD30 & has been published & validated for use in FC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Biochem/physiol Actions
Antibody recognizes Human CD30 antigen.
Physical form
Protein A purified
Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Preparation Note
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Jurkat cells
Evaluated by flow cytometry using Jurkat cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | FITC conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | Ber-H2, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001234 |
| UniProt accession no. | P28908 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TNFRSF8(943) |
| 长度(mm) | |
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