- 商品介绍
- 规格参数
- 包装参数
General description
40-44 kDa observed
Polycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.
Immunogen
Epitope: a.a. 1-202
Recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.
This Anti-Bmi-1 Antibody, clone F6, Alexa Fluor™ 488 is validated for use in Immunocytochemistry for the detection of Bmi-1.
Physical form
Protein G Purified
Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HepG2 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | F6, monoclonal |
| species reactivity | human, monkey |
| species reactivity (predicted by homology) | rabbit (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_031578.2 |
| UniProt accession no. | P25916 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... BMI1(648) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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¥ 1451.54
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¥ 2068.25
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¥ 6330.00
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¥ 2307.00
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NALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/8 x 1/4 x 1/16，英寸，每箱数量50，50/箱，8000-0020，Thermofisher，赛默飞世尔
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