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General description
Protein phosphatase 1A, also known as Protein phosphatase 2C isoform alpha (PP2C-alpha) or Protein phosphatase IA, and encoded by the gene name PPM1A, is a member of the PP2C family. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.
~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified recombinant fragment of human PPM1A expressed in E. Coli
Application
Anti-PPM1A, clone 7F12 Antibody is an antibody against PPM1A for use in western blotting, flow cytometry & ELISA.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PPM1A in HeLa cells.
ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Signaling
Research Sub Category
Metabolic Hormones & Receptors
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PPM1A in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 7F12, monoclonal |
| species reactivity | human |
| technique(s) | ELISA: suitable,flow cytometry: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P35813 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PPM1A(5494) |
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