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General description
The InsP3 (inositol 1,4,5-trisphosphate) Receptor family are intracellular Ca2+ channels activated by the second messenger InsP3. There are at least three types of receptor, with distinct tissue-specific expression profiles and subcellular localizations; it is thought that the exact subtype profile conveys precise Ca2+ release responses. Release of Ca2+ from intracellular stores is the trigger for many cellular responses, including muscle contraction. Receptor activity is subject to feedback regulation by Ca2+ concentration, as high cytosolic Ca2+ concentrations shut down the channel. Ca2+ release is augmented by phosphorylation of the Receptor by PKA and PKG.
~260 kDa
Immunogen
Epitope: C-terminus
Synthetic peptide (KDSTEYTGPESYV) corresponding to the C-terminus of human IP3 Receptor type 1.
Application
Immunohistochemisty (paraffin): IP3 Receptor staining in rat cerebellum, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select
Immunofluorescence: 1:20 - 1:200 dilution from a previous lot. Light fixation, 2% PFA, 90% ethanol fixation; Visualization by confocal microscopy is required, as detection by standard fluorescent microscopy will not be adequate to detect the IP3R. Due to the intensity of confocal lasers, use of an anti-fading agent, such as DABCO, is strongly recommended.
ELISA: 1:2,000 dilution of a previous was used in ELISA.
Immunoprecipitation: 1:200 dilution of a previous lot was used in Immunoprecipitation.
Flow cytometry: 1:1,000 dilution of a previous lot was used in Flow Cytometry.
Radioimmunoassay: 1:10,000 dilution of a previous lot was used in Radioimmunoassay.
Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-IP3 Receptor Antibody is validated for use in WB, IF, IH(P), ELISA, IP, RIA for the detection of IP3 Receptor.
Biochem/physiol Actions
Reactivity with other species has not been determined.
Reacts with the C-terminal cytoplasmic domain of IP3 receptors Type 1, 2, and 3. IP3 receptors have been shown to play an important role in the regulation of Ca2+ release from internal storage sites during cell activation. AB1622 reacts with IP3 receptor (MW approx. 260 kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).
Physical form
Format: Purified
Purified
Purified rabbit polyclonal IgG in buffer containing 10 mM sodium phosphate, 150 mM NaCl, and 0.01% sodium azide, pH 7.5.
Preparation Note
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
RAW264 cell lysate
Routinely evaluated by Western Blot on RAW 264 lysates.
Western Blot Analysis:
1:500-1,000 dilution of this lot detected IP3 Receptor on 10 μg of RAW 264 lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB1622
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | pig, human, bovine, rabbit, mouse, canine, dog, rat |
| technique(s) | ELISA: suitable,immunofluorescence: suitable,immunohistochemistry: suitable (paraffin),immunoprecipitation (IP): suitable,radioimmunoassay: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_001099952.1 |
| UniProt accession no. | Q14643 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITPR1(3708) |
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| 重量(kg) |
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