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- 规格参数
- 包装参数
General description
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-termial glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5.
~25 kDa observed
Immunogen
His-tagged recombinant protein corresponding to human UCHL1.
Application
Detect UCH-L1 using this mouse monoclonal antibody, Anti-UCHL1 Antibody, clone MCA-BH7 validated for use in western blotting & Immunofluorescence.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected UCHL1 in 10 µg of human brain tissue lysate.
Immunofluorescence Analysis: A 1:2,000 dilution from a representative lot detected UCHL1 in rat brain spinal cord tissue (Gerry Shaw. EnCor Biotechnology, Inc., Gainesville, Florida).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Rat brain spinal cord tissue lysate.
Evaluated by Western Blotting in rat brain spinal cord tissue lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected UCHL1 in 10 µg of rat brain tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | MCA-BH7, monoclonal |
| species reactivity | human, bovine, rat |
| technique(s) | immunofluorescence: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_004172 |
| UniProt accession no. | P09936 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UCHL1(7345) |
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