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BioTracker NucView® 488 Green Caspase-3 Dye (DMSO)，100UL，SCT100，Sigma

销售价： ¥ 6041.45 / 件

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订货号： 0BV4880

品牌型号： Sigma SCT100

货期：询货期

最小订货量： 1件

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商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

Caspases are a family of protease enzymes that play essential roles in programmed cell death, termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.

The BioTracker NucView 488 Green Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, the BioTracker NucView 488 Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence. Thus, NucView 488 Caspase-3 Substrate is bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The dye is formaldehyde-fixable and compatible with subsequent immunostaining. The dye is offered in DMSO and PBS (phosphate-buffered saline) formulations. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity.

Spectral Properties
Absorbance: 500nm
Emission: 530nm

Application

Live cell fluorescent imaging
Live cell imaging apoptosis dye for caspase-3/7 enzyme activity used to detect programmed cell death using fluorescence microscopy or flow cytometry applications.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Physical form

Liquid

Preparation Note

Store BioTracker NucView 488 Green Caspase-3 Dye, 1 mM in DMSO at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Analysis Note

Spectral Properties
Absorbance: 500nm
Emission: 530nm

Legal Information

NucView is a registered trademark of Biotium Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

technique(s)	cell based assay: suitable
detection method	fluorometric
shipped in	ambient
storage temp.	2-8°C

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