Anti-MHC class II (I-A/I-E), violetFluor® 450, clone M5/114 .15.2 Antibody,100UG,MABF1413,Sigma

销售价: ¥ 3875.14 / 件
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订货号 0BV4458
品牌型号 Sigma MABF1413
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.


Immunogen

Immunogen information for this antibody is proprietary information.


Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, violetFluor® 450, clone M5/114 .15.2, Cat. No. MABF1413 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).


Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography


Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.


Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected MHC class II (I-A/I-E) in one million C57Bl/6 splenocytes.


Other Notes

Concentration: Please refer to lot specific datasheet.


Legal Information

violetFluor is a registered trademark of Tonbo Biotechnologies


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerat
Quality Segment100
conjugatevioletFluor® 450
antibody formpurified antibody
antibody product typeprimary antibodies
cloneM5/114.15.2, monoclonal
species reactivitymouse
packagingantibody small pack of 25 μg
technique(s)flow cytometry: suitable
isotypeIgG2bκ
UniProt accession no.P04441
target post-translational modificationunmodified
Gene Informationmouse ... Cd74(16149)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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