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General description
Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.
Immunogen
Bovine myocardial m-calpain.
Epitope: small subunit of μ- or m-Calpains (Calpain I or II)
Application
Immunoblot: 1:500-1,000. The small subunit of either μ-calpain m-calpain is 30kDa. ELISA: 1:50,000 (against the immunogen). Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Optimal working dilutions must be determined by the end user.
This Anti-Calpain Antibody, small subunit of μ-or m-Calpains (Calpain I or II), clone P1 is validated for use in ELISA, WB, IC, IH for the detection of Calpain.
Biochem/physiol Actions
Reacts with the small subunit of human erthrocyte μ-calpain (Calpain I) or bovine myocardial m-calpain (Calpain II).
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | P1, monoclonal |
| species reactivity | human, bovine |
| should not react with | rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_001003962.1,NM_001749.2 |
| UniProt accession no. | P04632 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CAPNS1(826) |
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¥ 1302.89
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¥ 5725.45
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¥ 1653.62
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¥ 1156.91
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¥ 4011.97
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¥ 2277.64
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¥ 56581.65
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¥ 14985.93
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