Anti-LAG3 Antibody, clone 8C7.1，100UL，MABF294，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Lymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.
~60 kDa observed

Immunogen

Mouse Fc-tagged recombinant human LAG3 extracellular fragment.

Application

Flow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.
This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.

Physical form

Mouse monoclonal IgG2aκ antibody tissue culture supernatant with 0.05% sodium azide.

Analysis Note

Evaluated by Western Blotting in NK-92c ell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

技术参数 Specifications

biological source	mouse
Quality Segment	100
conjugate	unconjugated
antibody form	culture supernatant
antibody product type	primary antibodies
clone	8C7.1, monoclonal
species reactivity	human
technique(s)	flow cytometry: suitable,western blot: suitable
isotype	IgG2aκ
NCBI accession no.	NP_002277
UniProt accession no.	P18627
shipped in	dry ice
target post-translational modification	unmodified
Gene Information	human ... LAG3(3902)

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