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General description
PPIase D is one of a family peptidyl-prolyl-isomerases that help proteins fold during synthesis. Specifically, PPIases D catalyzes the cis-trans isomerization of proline imidic peptide bonds in peptides. Among the proteins assisted by PPIase D are HSP90, Estrogen receptor, cellular dynein, MYB, ILF2, XRCC6, GNB2L1 and RPS3. PPIase D also may regulate the interactions of other proteins including MYB and AHR. Through its protein folding exercise PPIase D becomes vital for proper protein transport and expression as well. Finally PPIase D is associated with assisting in the response to UV light induced apoptosis, cell viability in anaplastic lymphoma and hepatitis C virus replication and release. PPIase can be found localized in both the cytoplasm and the nucleus and is widely expressed. Note PPIase should not be confused with PPIF, or mitochondrial peptidyl-prolyl cis-trans isomerase F.
~ 42 kDa observed
Immunogen
Recombinant protein corresponding to human PPID.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PPID in rat cerebellum tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This Anti-PPID Antibody, clone 6A5.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of PPID.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected PPID in 10 µg of HepG2 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 6A5.1, monoclonal |
| species reactivity | rat, human |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_005029 |
| UniProt accession no. | Q08752 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PPID(5481) |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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