Anti-2,2,7-Trimethylguanosine Antibody, clone K121，100UG，MABE302，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

The 2,2,7-trimethylguanosine cap structure is found on the 5’ end of small nuclear RNAs and small nucleolar RNAs. Initially, these RNA molecules are capped with 7-monomethylguanosine (m7G) which is then methylated by the trimethylguanosine synthase enzyme to form 2,2,7-trimethylguanosine. The synthesis of 2,2,7-trimethylguanosine occurs in the cytoplasm and is an important marker for nuclear localization of RNA molecules. Capping of RNAs is an important requirement for RNA transport and splicing processes. Uncapped RNAs are rapidly degraded by the 5′ exoribonuclease enzyme. 2,2,7-trimethylguanosine capping may play a role in the expression of viral RNAs.

Application

Anti-2, 2, 7-Trimethylguanosine Antibody, clone K121 is a high quality Mouse Monoclonal Antibody for the detection of 2, 2, 7-Trimethylguanosine & has been validated in ICC, PAI & IP.
Immunoaffinity Purification Analysis: A representative lot from an independent laboratory was used in IAP (Krainer, A.R., et al. (1988). Nucleic Acids Res. 16(20):9415-9429.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory was immunoprecipitated in IP (Moketi, S., et al. (2002). Mol Cell. 10(3):599-609.).

Physical form

Format: Purified

Analysis Note

Control
NIH/3T3, HeLa, and A431 cells
Evaluated by Immunocytochemistry in NIH/3T3, HeLa, and A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected 2,2,7-trimethylguanosine in NIH/3T3, HeLa, and A431 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

技术参数 Specifications

biological source	mouse
Quality Level	100
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	K121, monoclonal
species reactivity	human, mouse
technique(s)	affinity chromatography: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable
isotype	IgG1κ
shipped in	wet ice
target post-translational modification	unmodified

长度(mm)
宽度(mm)
高度(mm)
重量(kg)